Diffusion of glycophorin A in human erythrocytes
نویسندگان
چکیده
منابع مشابه
Molecular analysis of glycophorin C deficiency in human erythrocytes.
Human erythrocyte glycophorin C plays a functionally important role in maintaining erythrocyte shape and regulating membrane mechanical stability. We report here the characterization of the glycophorins C and D deficiency in erythrocytes of the Leach phenotype. Glycophorin C gene is encoded by 4 exons. Amplification of reticulocyte cDNA from Leach phenotype and normal individuals generated a 14...
متن کاملGlycophorin and the concanavalin A receptor of human erythrocytes: their receptor function in lipid bilayers.
Two integral glycoproteins from the human erythrocyte have been studied after their incorporation into lipid bilayer systems. Glycophorin (which is the M/N blood group determinant) and the concanavalin A receptor were isolated and purified prior to incorporation into model membranes by dialytic removal of detergent from lipid/protein solutions. Under the conditions described, glycoprotein recep...
متن کاملSomatic Cell Mutation Assay at the Glycophorin-a Locus in Human Erythrocytes for Biodosimetry
For risk assessment, it is necessary to evaluate the dose of human exposure to radiation. Ionizing radiation being an environmental genotoxic factor induces dosedependent somatic mutations. Recent studies support the somatic-mutation theory of carcinogenesis. Mutations at the Glycophorin–A (GPA) loci have been studied for establishment of somatic mutation assays. GPA assay provides a lifetime b...
متن کاملAbnormality of glycophorin-alpha on paroxysmal nocturnal hemoglobinuria erythrocytes.
To investigate the greater enzymatic activity of the alternative pathway convertase (and the subsequent greater fixation of C3b) on paroxysmal nocturnal hemoglobinuria (PNH) erythrocytes, we have examined the topography of binding of C3b to PNH and normal erythrocytes. Using sodium dodecyl sulfate-polyacrylamide gel electrophoresis and autoradiography, the alpha-chain of C3b was found to bind v...
متن کاملGlycophorin A interferes in the agglutination of human erythrocytes by concanavalin A. Explanation of the requirement for enzymic predigestion.
Human erythrocytes become agglutinable with concanavalin A (Con A) after treatment with various proteinases or neuraminidase. The extent of agglutinability achieved with different enzymes is, however, different: Pronase, papain, trypsin, neuraminidase and chymotrypsin enhance the agglutinability in decreasing order, the last being barely effective. The actions of the enzymes on band 3, the Con ...
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ژورنال
عنوان ژورنال: Biochimica et Biophysica Acta (BBA) - Biomembranes
سال: 2016
ISSN: 0005-2736
DOI: 10.1016/j.bbamem.2016.08.012